GALT Rabbit Polyclonal Antibody

SKU: orb402464

Description

Anti-GALT Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Metabolism Research, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC, IP, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry(Fixed), 1-3 μg/1x10^6 cells ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human GALT recombinant protein (Position: Q188-A379).
• Target: Galactose-1-phosphate uridylyltransferase
• Molecular Weight: 48 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Galactose-1-phosphate uridylyltransferase; Gal-1-P uridylyltransferase; 2.7.7.12; UDP-glucose--hexose-1-phosphate uridylyltransferase; GALT

Flow Cytometry analysis of HepG2 cells using anti-GALT antibody. Overlay histogram showing HepG2 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GALT Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

IHC analysis of GALT using anti-GALT antibody. GALT was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-GALT Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Immunoprecipitating GALT in THP-1 whole cell lysate. Western blot analysis of GALT using anti-GALT antibody; Lane 1: THP-1 whole cell lysates (30 ug); Lane 2: Rabbit control IgG instead of anti-GALT antibody in THP-1 whole cell lysate; Lane 3: anti-GALT antibody (2 µg) + THP-1 whole cell lysate (500 µg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GALT antigen affinity purified polyclonal antibody at a dilution of 0.5 µg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate. A specific band was detected for GALT at approximately 43 kDa. The expected band size for GALT is at 43 kDa.

Western blot analysis of GALT using anti-GALT antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GALT antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GALT at approximately 43 kDa. The expected band size for GALT is at 43 kDa.

Quick Database Links

Gene Symbol

Galactose-1-phosphate uridylyltransferase

UniProt

P07902