GABA Transporter 1/GAT 1/SLC6A1 Rabbit Polyclonal Antibody

SKU: orb402560

Description

Anti-GABA Transporter 1/GAT 1/SLC6A1 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cell Biology, Neuroscience

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat Immunofluorescence, 5 μg/ml, Human
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A1, different from the related mouse and rat sequences by two amino acids.
• Target: Sodium- and chloride-dependent GABA transporter 1
• Molecular Weight: 67 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sodium- and chloride-dependent GABA transporter 1; GAT-1; Solute carrier family 6 member 1; SLC6A1; GABATR, GABT1, GAT1

Western blot analysis of SLC6A1 using anti-SLC6A1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC6A1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC6A1 at approximately 88KD. The expected band size for SLC6A1 is at 67KD.

Quick Database Links

Gene Symbol

Sodium- and chloride-dependent GABA transporter 1

UniProt

P30531