FSHR Rabbit Polyclonal Antibody

SKU: orb213952

Description

The FSHR Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FSHR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FSHR. The exact sequence is proprietary.
• Target: FSHR
• Molecular Weight: 78 kDa
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LGR1; Follicle-stimulating hormone receptor; FSH-R; Follitropin receptor

Immunofluorescent analysis of FSHR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Western blot analysis of rat heart (1), rat testis (2) using FSHR Rabbit Polyclonal Antibody orb213952 with dilution at 1:1000.

Immunohistochemical analysis of paraffin-embedded mouse heart using FSHR Rabbit Polyclonal Antibody orb213952 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded mouse testis using FSHR Rabbit Polyclonal Antibody orb213952 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat kidney using FSHR Rabbit Polyclonal Antibody orb213952 with dilution at 1:100.

Quick Database Links

Gene Symbol

FSHR

UniProt

P23945, P35378, P20395

NCBI Gene ID

2492, 25449, 14309

Xing, Yu et al. Effects of Gui Zhu Yi Kun formula on the P53/AMPK pathway of autophagy in granulosa cells of rats with polycystic ovary syndrome Exp Ther Med, 13, 3567-3573 (2017)

PubMed: 28588681

Applications

IHC

Reactivity

Rat

Zhou, Ge et al. Tyrosine nitrations impaired intracellular trafficking of FSHR to the cell surface and FSH-induced Akt-FoxO3a signaling in human granulosa cells Aging (Albany NY), 11, 3094-3116 (2019)

PubMed: 31097679

Applications

IB

Reactivity

Human

Wang, Jiawei et al. Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in Chemotherapy-Induced POF Rats Stem Cells Int, 2021, 8293699 (2021)

PubMed: 34733331

Applications

IHC

Reactivity

Rat