Anti-FSHR Antibody

• Catalog Number: orb213952
• Category: Antibodies
• Description: Rabbit polyclonal antibody to FSHR
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FSHR. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FSHR
• Entrez: 25449, 14309, 2492
• UniProt ID: P20395, P35378, P23945
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti LGR1 antibody, anti Follicle-stimulating horm
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Wang, Jiawei et al. Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in Chemotherapy-Induced POF Rats Stem Cells Int, 2021, 8293699 (2021)

PubMed: 34733331

Applications

IHC

Reactivity

Rat

Xing, Yu et al. Effects of Gui Zhu Yi Kun formula on the P53/AMPK pathway of autophagy in granulosa cells of rats with polycystic ovary syndrome Exp Ther Med, 13, 3567-3573 (2017)

PubMed: 28588681

Applications

IHC

Reactivity

Rat

Zhou, Ge et al. Tyrosine nitrations impaired intracellular trafficking of FSHR to the cell surface and FSH-induced Akt-FoxO3a signaling in human granulosa cells Aging (Albany NY), 11, 3094-3116 (2019)

PubMed: 31097679

Applications

IB

Reactivity

Human

Western blot analysis of FSHR expression in SKOVCAR3 (A), rat testis (B), A2780 (C) whole cell lysates. (Predicted band size: 78 kD; Observed band size: 71; 75; 81 kD)

Immunohistochemical analysis of FSHR staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of FSHR staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.