FOXC1 Rabbit Polyclonal Antibody

SKU: orb570383

Description

Anti-FOXC1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Disease Biomarkers, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Dilution Range: Western blot, 0.1-0.25μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human ELISA, 0.1-0.5μg/ml, -
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human FOXC1 recombinant protein (Position: T392-F554).
• Target: Forkhead box protein C1
• Molecular Weight: 75 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARA; FKHL7; forkhead box C1; Forkhead box protein C1; Forkhead related protein FKHL7; FOXC1; FREAC 3; FREAC3; IGDA; IHG1; IRID1; RIEG3

IF analysis of FOXC1 using anti-FOXC1 antibody and anti-Tubulin Alpha antibody. FOXC1 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-FOXC1 Antibody and mouse anti-Tubulin Alpha antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Western blot analysis of FOXC1 using anti-FOXC1 antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FOXC1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate with Tanon 5200 system. A specific band was detected for FOXC1 at approximately 75 kDa. The expected band size for FOXC1 is at 53 kDa.

Quick Database Links

Gene Symbol

Forkhead box protein C1

UniProt

Q12948