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Anti-FKBP135/FKBP15 Antibody

Catalog Number: orb865516

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb865516
CategoryAntibodies
DescriptionAnti-FKBP135/FKBP15 Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, ICC, IF, IHC, WB
ReactivityHuman, Mouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived human FKBP135/FKBP15 recombinant protein (Position: A19-D1210).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW160 kDa
UniProt IDQ5T1M5
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesE3 ubiquitin-protein ligase RNF169; RING finger pr
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NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-FKBP135/FKBP15 Antibody

IHC analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody. FKBP135/FKBP15 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-FKBP135/FKBP15 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-FKBP135/FKBP15 Antibody

Flow Cytometry analysis of JK cells using anti-FKBP135/FKBP15 antibody. Overlay histogram showing JK cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FKBP135/FKBP15 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Anti-FKBP135/FKBP15 Antibody

IF analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody. FKBP135/FKBP15 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-FKBP135/FKBP15 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-FKBP135/FKBP15 Antibody

IHC analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody. FKBP135/FKBP15 was detected in a paraffin-embedded section of human breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-FKBP135/FKBP15 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-FKBP135/FKBP15 Antibody

IHC analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody. FKBP135/FKBP15 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-FKBP135/FKBP15 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-FKBP135/FKBP15 Antibody

IHC analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody. FKBP135/FKBP15 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-FKBP135/FKBP15 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-FKBP135/FKBP15 Antibody

IHC analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody. FKBP135/FKBP15 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-FKBP135/FKBP15 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-FKBP135/FKBP15 Antibody

IHC analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody. FKBP135/FKBP15 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-FKBP135/FKBP15 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-FKBP135/FKBP15 Antibody

Western blot analysis of FKBP135/FKBP15 using anti-FKBP135/FKBP15 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FKBP135/FKBP15 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FKBP135/FKBP15 at approximately 160 kDa. The expected band size for FKBP135/FKBP15 is at 160 kDa.

  • Anti-FKBP135/FKBP15 Antibody [orb2599232]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-FKBP135/FKBP15 Antibody [orb2599233]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-FKBP135/FKBP15 Antibody [orb2599234]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    APC

    100 μg
  • Anti-FKBP135/FKBP15 Antibody [orb2599235]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    HRP

    100 μg
  • Anti-FKBP135/FKBP15 Antibody [orb2599236]

    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    FITC

    100 μg