Anti-Estrogen Inducible Protein pS2/TFF1 Antibody

• Catalog Number: orb308817
• Category: Antibodies
• Description: Anti-Estrogen Inducible Protein pS2/TFF1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Immunogen: E. coli-derived human TFF1 recombinant protein (Position: E25-F84). Human TFF1 shares 66.7% and 69% amino acid (aa) sequence identity with mouse and rat TFF1, respectively.
• UniProt ID: P04155
• MW: 12 kDa
• Tested applications: ELISA, WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human, -ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Trefoil factor 1; Breast cancer estrogen-inducible
• Note: For research use only

Western blot analysis of TFF1 using anti-TFF1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: HELA Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFF1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TFF1 at approximately 12 kDa. The expected band size for TFF1 is at 12 kDa.