Anti-ERP57

• Catalog Number: orb11607
• Category: Antibodies
• Description: Goat polyclonal to ERp57 - endoplasmic reticulum lumen marker. This endoplasmic reticulum protein interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. It has disulfide isomerase activity and complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Reactivity: Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
• Isotype: IgG
• Immunogen: Antigen: Purified recombinante peptide derived from within residues 300 aa to the C-terminus of human ERP57 produced in E. coli.. Antigen Sequence: STAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRESDFISYLQREATNPPVIQEEKPKKKKKAQEDL
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: WB:1:500-1:2,000, IF:1:50-1:500, IHC-P:1:200-1:1,000, IHC-F:1:200-1:1,000
• Conjugation: Unconjugated
• Target: PDIA3 protein disulfide isomerase family A, member 3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Alternative names: 58 kDa glucose regulated protein, 58 kDa microsoma
• Note: For research use only
• Application notes: The antibody solution should be gently mixed before use.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Melan-ink and RPE19 cell lysates using ERP57 antibody

Confocal immunofluorescence analysis of RPE cells using ERP57 antibody