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ERK1/MAPK3 Rabbit Polyclonal Antibody
Description
Research Area
Images & Validation
−| Tested Applications | ICC, IHC, WB |
|---|---|
| Dilution Range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse Immunocytochemistry, 0.5-1μg/ml, Human, Mouse, Rat Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ERK1, identical to the related mouse and rat sequences. |
| Target | Mitogen-activated protein kinase 3 |
| Molecular Weight | 100 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunocytochemical staining of paraffin embedded MCF-1 cell using GAPDH antibody
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ERK1/MAPK3 Rabbit Polyclonal Antibody (orb27557)
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