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Anti-EPHB1/2 (Phospho-Y594/604) Antibody
Catalog Number: orb224166
| Catalog Number | orb224166 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to EPHB1/2 (Phospho-Y594/604) |
| Target | EPHB1; EPHB2 |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Buffer/Preservatives | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y594/604 of human EPHB1/2 protein. The exact sequence is proprietary. |
| UniProt ID | P29323, P54763, Q8CBF3, P54762, P09759 |
| Tested applications | IF, WB |
| Dilution range | WB: 1:500-1:1000, IF/ICC: 1:100-1:500 |
| Antibody Type | Primary Antibody |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti EPHB1 antibody, anti ELK antibody, anti EPHT2 Read more... |
| Note | For research use only |
| Entrez | 24338, 2047, 1969, 270190 |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of EPHB1/2 (Phospho-Y594/604) expression in A431 (A), HeLa (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 109; 117 kD; Observed band size: 130 kD)
Immunofluorescent analysis of EPHB1/2 (Phospho-Y594/604) staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Direct ELISA antibody dose-response curve using Anti-EPHB1/2 (Phospho-Y594/604) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
