Eotaxin/Ccl11 Antibody

• Catalog Number: orb1098112
• Category: Antibodies
• Description: Anti-Eotaxin/Ccl11 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Rat.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E.coli-derived rat Eotaxin/Ccl11 recombinant protein (Position: H24-P97).
• UniProt ID: P97545
• MW: 75 kDa
• Tested applications: ELISA, IHC
• Application notes: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat ELISA, 0.1-0.5 μg/ml, Rat. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Cross Reactivity: No cross-reactivity with other proteins.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Bestrophin-1; TU15B; Vitelliform macular dystrophy
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC analysis of Eotaxin/Ccl11 using anti-Eotaxin/Ccl11 antibody. Eotaxin/Ccl11 was detected in a paraffin-embedded section of rat lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Eotaxin/Ccl11 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.