Anti-EEA1

• Catalog Number: orb11614
• Category: Antibodies
• Description: Goat polyclonal antibody to EEA1 – early endosome marker. EEA1 is Early Endosome Antigen 1 protein that binds phospholipid vesicles containing phosphatidylinositol 3-phosphate, which is necessary for endosomal trafficking.
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Bovine, Canine, Donkey, Equine, Feline, Gallus, Goat, Guinea pig, Hamster, Human, Mouse, Other, Porcine, Primate, Rabbit, Rat, Sheep
• Isotype: IgG
• Immunogen: Antigen: Purified recombinant peptide within residues 1,230 aa to the C-terminus of human EEA1 produced in E. coli.. Antigen Sequence: KKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: WB:1:250-1:1,000, IF:1:50-1:250
• Conjugation: Unconjugated
• Target: Early Endosome Antigen 1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 20% glycerol and 0.05% sodium azide
• Alternative names: Early endosome antigen 1, 162kD; early endosome-as
• Note: For research use only
• Application notes: The antibody solution should be gently mixed before use.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of At-T20 cell lysate using EEA1 antibody

Confocal immunofluorescence analysis of Hepa cell using EEA1 antibody