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Anti-ECA39 Antibody

Catalog Number: orb213600

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb213600
CategoryAntibodies
DescriptionRabbit polyclonal antibody to BCAT1
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Porcine, Primate, Rabbit, Rat, Sheep
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human ECA39. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1-500-1-1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetBCAT1
Entrez586, 29592, 12035
UniProt IDP54687, P24288, P54690
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti BCT1 antibody, anti ECA39 antibody, anti Bran
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-ECA39 Antibody

Western blot analysis of ECA39 expression in Jurkat (A), Ramos (B), NIH3T3 (C), PC12 (D) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)

Anti-ECA39 Antibody

Immunofluorescent analysis of ECA39 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

  • Anti-ECA39 Antibody [orb319061]

    IH,  WB

    Human, Mouse, Primate, Rabbit, Sheep

    Rabbit

    Polyclonal

    Unconjugated

    200 μl, 100 μl, 30 μl
  • ECA39 rabbit pAb [orb767048]

    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • BCAT1 Antibody [orb672238]

    ELISA,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
  • ECA39 Antibody [orb653220]

    WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • BCAT1 Antibody [orb677020]

    ELISA,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl