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Anti-EAAT1/SLC1A3 Antibody

Catalog Number: orb371693

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb371693
CategoryAntibodies
DescriptionAnti-EAAT1/SLC1A3 Antibody. Tested in WB applications. This antibody reacts with Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsWB
ReactivityMouse, Rat
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the N-terminus of human EAAT1, different from the related mouse sequence by three amino acids, and identical to the related rat sequence.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW60 kDa
UniProt IDP43003
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesExcitatory amino acid transporter 1; Sodium-depend
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NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml, Mouse, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-EAAT1/SLC1A3 Antibody

Western blot analysis of EAAT1/SLC1A3 using anti-EAAT1/SLC1A3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EAAT1/SLC1A3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EAAT1/SLC1A3 at approximately 60 kDa. The expected band size for EAAT1/SLC1A3 is at 60 kDa.

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    WB

    Human, Mouse, Rat

    Rabbit

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    ELISA,  FC,  WB

    Human, Mouse, Rat

    Rabbit

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    100 μg, 10 μg
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    IF,  IHC,  WB

    Mouse, Rat

    Rabbit

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