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Anti-DRP1/DNM1L Antibody

Catalog Number: orb1147777

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb1147777
CategoryAntibodies
DescriptionAnti-DRP1/DNM1L Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived human DRP1/DNM1L recombinant protein (Position: Q452-W736).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW75 kDa
UniProt IDO00429
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesReceptor-type tyrosine-protein phosphatase C; Leuk
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NoteFor research use only
Application notesWestern blot, 0.25-0.5 μg/ml, Human, Rat ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
Expiration Date12 months from date of receipt.
Anti-DRP1/DNM1L Antibody

Western blot analysis of DRP1/DNM1L using anti-DRP1/DNM1L antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRP1/DNM1L antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DRP1/DNM1L at approximately 75 kDa. The expected band size for DRP1/DNM1L is at 82 kDa.

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    ELISA,  FC,  ICC,  IF,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
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    ELISA,  WB

    Human, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg
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    ELISA,  WB

    Human, Rat

    Rabbit

    Polyclonal

    PE

    100 μg
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    ELISA,  WB

    Human, Rat

    Rabbit

    Polyclonal

    APC

    100 μg
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    ELISA,  WB

    Human, Rat

    Rabbit

    Polyclonal

    HRP

    100 μg