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Anti-DRAGON/RGMB Antibody
Catalog Number: orb669119
| Catalog Number | orb669119 |
|---|---|
| Category | Antibodies |
| Description | Anti-DRAGON/RGMB Antibody. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, WB |
| Reactivity | Human, Mouse |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human DRAGON/RGMB recombinant protein (Position: D47-R404). |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 48 kDa |
| UniProt ID | Q6NW40 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Aflatoxin B1 aldehyde reductase member 2; AFB1 ald Read more... |
| Note | For research use only |
| Application notes | Western blot, 0.25-0.5μg/ml, Human, Mouse Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human ELISA, 0.1-0.5μg/ml, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of A431 cells using anti-DRAGON/RGMB antibody. Overlay histogram showing A431 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DRAGON/RGMB Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Western blot analysis of DRAGON/RGMB using anti-DRAGON/RGMB antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRAGON/RGMB antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DRAGON/RGMB at approximately 48 KD. The expected band size for DRAGON/RGMB is at 48 KD.
