Anti-DR5 [304]

• Catalog Number: orb1463374
• Category: Antibodies
• Description: Human monoclonal antibody to DR5
• Target: DR5
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: IgG4-S228P
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: The antibody was raised in mice homozygous for both disrupted endogenous Ig heavy chain and k light chain, harboring a chromosome 14 fragment (SC20) containing a human Ig heavy chain locus and a human Igk chain transgene (KCo5). The mice were immunised against TRAIL-R1 and TRAIL-R2.
• UniProt ID: O14763
• Tested applications: ELISA, RIA
• Clone Number: 304
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TRAILR2; TNFRSF10B; TRAIL receptor 2; tumor necros
• Research Area: Cancer Biology, Cell Biology, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-DR5 antibody 304. Caco-2 cells were stained with an isotype control (orb256390, black line) or the rabbit-chimeric version of 304 (orb1463373, blue line) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488® conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescence staining of Caco-2 cells with anti-DR5 304. Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric rabbit IgG version of 304 (orb1463373) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463373, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-DR5 antibody 304. HeLa(A) (0.5 µg/ml), Caco-2(B) (0.5 µg/ml) and K562(C) (0.5 µg/ml) cells lysate tissue lysate (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 304 (orb1463373) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.