Dnmt3L Antibody

• Catalog Number: orb340835
• Category: Antibodies
• Description: The Dnmt3L Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human Dnmt3L .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Recombinant fusion protein of human Dnmt3L
• UniProt ID: Q9UJW3, Q1LZ50, Q9CWR8
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1:2000, IF/ICC: 1:20-1:50, IP: 1:20-1:50, ChIP: 1:20-1:50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DNA cytosine-5)-methyltransferase 3-like
• Note: For research use only
• Entrez: 309680, 29947, 54427
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Dnmt3L expression in PC3 (A), Jurkat (B), MCF7 (C) whole cell lysates. (Predicted band size: 43 kD; Observed band size: 43 kD)

Immunohistochemical analysis of Dnmt3L staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.