Anti-DNMT3A Antibody

• Catalog Number: orb234826
• Category: Antibodies
• Description: Rabbit polyclonal antibody to DNMT3A
• Target: DNMT3A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DNMT3A. The exact sequence is proprietary.
• UniProt ID: Q1LZ53, Q9Y6K1, O88508
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DNA (cytosine-5)-methyltransferase 3A antibod
• Note: For research use only
• Entrez: 1788, 444984, 13435

Western blot analysis of DNMT3A expression in MEF (A), H9C2 (B), HCT116 (C), Jurkat (D), PC3 (E), HEK293T (F) whole cell lysates. (Predicted band size: 16; 81; 101 kD; Observed band size: 130 kD)

Immunohistochemical analysis of DNMT3A staining in human colorectal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.