Dnmt1 Rabbit Polyclonal Antibody

SKU: orb1290018

Description

Anti-Dnmt1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse.

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: Western blot, 0.25-0.5 μg/ml, Mouse ELISA, 0.1-0.5 μg/ml, -
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse Dnmt1 recombinant protein (Position: Q509-Q1577).
• Target: DNA
• Molecular Weight: 200 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AIM; CXXC9; DNA MTase HsaI; DNMT; DNMT1; MCMT

Western blot analysis of Dnmt1 using anti-Dnmt1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Dnmt1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Dnmt1 at approximately 200 kDa. The expected band size for Dnmt1 is at 183 kDa.

Quick Database Links

Gene Symbol

DNA

UniProt

P13864