Anti-DC-SIGNR [16E7]

• Catalog Number: orb348892
• Category: Antibodies
• Description: Rabbit monoclonal antibody to DC-SIGNR
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 16E7
• Tested applications: FC, IHC
• Reactivity: Human
• Isotype: Mouse IgG
• Immunogen: CD299
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: DC-SIGNR
• UniProt ID: Q9H2X3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD299; C-type lectin domain family 4 member M; CD2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluoresence staining of fixed HeLa cells with anti-DC-SIGNR antibody 16E7. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 16E7 (orb348892) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb348892, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-DC-SIGNR (CLEC4M) antibody 16E7 Jurkat cell extract (35 µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of 16E7 (orb348892) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for DC-SIGNR is 45.3kDa, though 9 other isoforms of this protein are known ranging in size from 22.4-44.7kDa (Uniprot ID: Q9H2X3). DC-SIGNR is also glycosylated at several positions. orb348892 successfully detected the canonical human DC-SIGNR, as well as multiple other isoforms.