Cytokeratin 1/Krt1 Antibody

• Catalog Number: orb1676498
• Category: Antibodies
• Description: Anti-Cytokeratin 1/Krt1 Antibody. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Mouse.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: E.coli-derived mouse Cytokeratin 1/Krt1 recombinant protein (Position: L223-Q439).
• UniProt ID: P04104
• MW: 66 kDa
• Tested applications: ELISA, IF, IHC, WB
• Application notes: Western blot, 0.25-0.5 μg/ml, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse Immunofluorescence, 5 μg/ml, Mouse ELISA, 0.1-0.5 μg/ml, -. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Cross Reactivity: No cross-reactivity with other proteins.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Pituitary homeobox 2; ALL1-responsive protein ARP1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IF analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody. Cytokeratin 1/Krt1 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL rabbit anti-Cytokeratin 1/Krt1 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody. Cytokeratin 1/Krt1 was detected in a paraffin-embedded section of mouse skin tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cytokeratin 1/Krt1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Western blot analysis of Cytokeratin 1/Krt1 using anti-Cytokeratin 1/Krt1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cytokeratin 1/Krt1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cytokeratin 1/Krt1 at approximately 66 kDa. The expected band size for Cytokeratin 1/Krt1 is at 66 kDa.