Anti-Cytokeratin 14 Antibody

• Catalog Number: orb214169
• Category: Antibodies
• Description: Rabbit polyclonal antibody to KRT14
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IH, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human Cytokeratin 14. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: KRT14; KRT16
• Entrez: 16664, 287701, 3861
• UniProt ID: Q61781, Q6IFV1, P02533
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti KRT14 antibody, anti Keratin, type I cytoskel
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Jin-Man Kim 1, Ho Kyoung Kim 1, Han Jin Cho 1, Sung-Ah Moon 2, Yewon Kim 2, Jeong Yeon Hong 3, Seung Hun Lee 4, Kyunggon Kim 5, Jung-Min Koh Extracellular C1qbp inhibits myogenesis by suppressing NFATc1 Sci Rep, (2024)

PubMed: 38977785

Applications

IHC

Reactivity

Mouse

Western blot analysis of Cytokeratin 14 expression in MCF7 (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 52 kD)

Immunohistochemical analysis of Cytokeratin 14 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Cytokeratin 14 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.