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Keratin, type I cytoskeletal 10 Antibody

SKU: orb2804049

Description

The Keratin, type I cytoskeletal 10 Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, ICC, IF, IHC, and WB applications. This antibody targets the Keratin, type I cytoskeletal 10 in Human and Mouse samples.

Images & Validation

Tested ApplicationsELISA, FC, ICC, IF, IHC, WB
Dilution RangeWestern blot, 0.25-0.5 μg/ml, Human, Mouse Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml
ReactivityHuman, Mouse
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenE.coli-derived human KRT10 recombinant protein (Position: N171-Q434).
TargetKeratin, type I cytoskeletal 10
Molecular Weight50-59 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

KRT10; KPP; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK-10; Keratin-10; K10

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ICC
Immunocytochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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