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Anti-Cytochrome P450 2E1/CYP2E1 Antibody

Catalog Number: orb215944

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb215944
CategoryAntibodies
DescriptionAnti-Cytochrome P450 2E1/CYP2E1 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIHC, WB
ReactivityMouse, Rat
IsotypeRabbit IgG
ImmunogenE.coli-derived human CYP2E1 recombinant protein (Position: M1-Y310). Human CYP2E1 shares 73% and 74% amino acid (aa) sequences identity with mouse and rat CYP2E1, respectively.
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW55 kDa
UniProt IDP05181
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesCytochrome P450 2E1; 1.14.13.-; 4-nitrophenol 2-hy
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NoteFor research use only
Application notesImmunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, HumanWestern blot, 0.1-0.5μg/ml, Mouse, Rat, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-Cytochrome P450 2E1/CYP2E1 Antibody

IHC analysis of CYP2E1 using anti-CYP2E1 antibody. CYP2E1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CYP2E1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Cytochrome P450 2E1/CYP2E1 Antibody

IHC analysis of CYP2E1 using anti-CYP2E1 antibody. CYP2E1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-CYP2E1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-Cytochrome P450 2E1/CYP2E1 Antibody

Western blot analysis of CYP2E1 using anti-CYP2E1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. lane 1: rat liver tissue lysate, lane 2: mouse liver tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CYP2E1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CYP2E1 at approximately 55 KD. The expected band size for CYP2E1 is at 57 KD.

  • Anti-Cytochrome P450 2E1/CYP2E1 Antibody [orb570377]

    ELISA,  FC,  IHC,  WB

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-Cytochrome P450 2E1/CYP2E1 Antibody [orb97035]

    IHC,  WB

    Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-Cytochrome P450 2E1/CYP2E1 Antibody (monoclonal, 2C7G1) [orb1145783]

    WB

    Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-Cytochrome P450 2E1/CYP2E1 Antibody [orb2623283]

    IHC,  WB

    Mouse, Rat

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-Cytochrome P450 2E1/CYP2E1 Antibody [orb2623284]

    IHC,  WB

    Mouse, Rat

    Rabbit

    Polyclonal

    PE

    100 μg