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Description
Research Area
Cancer Biology, Cardiovascular Research, Cell Biology, Metabolism Research, Protein Biochemistry, Signal Transduction
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat |
| Reactivity | Human, Mouse, Rat |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1, different from the related mouse and rat sequences by six amino acids. |
| Target | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial |
| Molecular Weight | 57 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial; 1.14.13.13; 25-OHD-1 alpha-hydroxylase; 25-hydroxyvitamin D (3) 1-alpha-hydroxylase; VD3 1A hydroxylase; Calcidiol 1-monooxygenase; Cytochrome P450 subfamily XXVIIB polypeptide 1; Cytochrome P450C1 alpha; Cytochrome P450VD1-alpha; Cytochrome p450 27B1; CYP27B1; CYP1ALPHA, CYP27B
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Quick Database Links
Gene Symbol
25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry













