Anti-Cyclin D1/CCND1 Antibody

• Catalog Number: orb180681
• Category: Antibodies
• Description: Anti-Cyclin D1/CCND1 Antibody. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Rat.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Cyclin D1, different from the related mouse and rat sequences by two amino acids.
• UniProt ID: P24385
• MW: 33 kDa
• Tested applications: FC, WB
• Application notes: Western blot, 0.1-0.5μg/ml, Human, Rat Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: G1/S-specific cyclin-D1; B-cell lymphoma 1 protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U-87MG cells using anti-Cyclin D1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cyclin D1 using anti-Cyclin D1 antibody.Lane 1:Rat Testis Tissue; 2:Human Placenta Tissue; 3:Rat Brain Tissue; 4:MCF-7 Cell; 5:COLO320 Cell; 6:SW620 Cell; 7:MM231 Cell.