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Catalog Number | orb1743882 |
---|---|
Category | Antibodies |
Description | Anti-CYC1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, FC, ICC, IF, WB |
Reactivity | Human |
Immunogen | E.coli-derived human CYC1 recombinant protein (Position: S85-K325). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.25-0.5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human Direct ELISA, 0.1-0.5 μg/ml, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 33 kDa |
UniProt ID | P08574 |
Storage | At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing. |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of MCF-7 cells using anti-CYC1 antibody(Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.
WB analysis of CYC1 using anti-CYC1 antibody.Lane 1:human A431 cell;2:human HeLa cell;3:human MCF-7 cell.
IF analysis of CYC1 using anti-CYC1 antibody. CYC1 was detected in an immunocytochemical section of A549 cells.
ELISA | |
Human | |
Rabbit | |
Polyclonal | |
Biotin |
Human | |
Rabbit | |
Polyclonal | |
FITC |
ELISA | |
Human | |
Rabbit | |
Polyclonal | |
HRP |
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