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Anti-CXCL16 Antibody
Catalog Number: orb381056
| Catalog Number | orb381056 |
|---|---|
| Category | Antibodies |
| Description | Anti-CXCL16 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human. |
| Clonality | Polyclonal |
| Species/Host | Rabbit |
| Isotype | Rabbit IgG |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Form/Appearance | Lyophilized |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Immunogen | E. coli-derived human CXCL16 recombinant protein (Position: N30-A196). Human CXCL16 shares 47% and 45.7% amino acid (aa) sequence identity with mouse and rat CXCL16, respectively. |
| UniProt ID | Q9H2A7 |
| MW | 35 kDa |
| Tested applications | ELISA, WB |
| Application notes | Western blot, 0.1-0.5μg/ml, Human ELISA(Cap) , 1-5μg/ml, Human, -. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | C-X-C motif chemokine 16; Scavenger receptor for p Read more... |
| Note | For research use only |
Sandwich ELISA - Recombinant human cxcl16 protein standard curve. Use in combination with reagents from Human cxcl16 ELISA Kit EZ-Set (DIY Antibody Pairs).
Western blot analysis of CXCL16 using anti-CXCL16 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: CEM whole cell lysates, Lane 2: A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCL16 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CXCL16 at approximately 35 kDa. The expected band size for CXCL16 is at 28 kDa.
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