CREB3L1 Antibody

SKU: orb308787

Description

Anti-CREB3L1 Antibody. Tested in WB applications. This antibody reacts with Human.

Images & Validation

• Tested Applications: WB
• Reactivity: Human
• Application Notes:
  Western blot, 0.1-0.5μg/ml, Human. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CREB3L1 recombinant protein (Position: M1-M204). Human CREB3L1 shares 93.6% and 93.1% amino acid (aa) sequence identity with mouse and rat CREB3L1, respectively.
• Target: Cyclic AMP-responsive element-binding protein 3-like protein 1
• Molecular Weight: 57 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Cyclic AMP-responsive element-binding protein 3-like protein 1; cAMP-responsive element-binding protein 3-like protein 1; Old astrocyte specifically-induced substance; OASIS; Processed cyclic AMP-responsive element-binding protein 3-like protein 1; CREB3L1; OASIS; PSEC0238

Western blot analysis of CREB3L1 using anti-CREB3L1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50 ug, Lane 2: HELA Whole Cell Lysate at 40 ug, Lane 3: A549 Whole Cell Lysate at 40 ug, Lane 4: MM231 Whole Cell Lysate at 40 ug, Lane 5: COLO320 Whole Cell Lysate at 40 ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CREB3L1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CREB3L1 at approximately 57 kDa. The expected band size for CREB3L1 is at 57 kDa.

Quick Database Links

Gene Symbol

Cyclic AMP-responsive element-binding protein 3-like protein 1

UniProt

Q96BA8

References

Buile, Dace et al. Evaluation of the Multiple Tissue Factors in the Cartilage of Primary and Secondary Rhinoplasty in Cleft Lip and Palate Patients Pediatr Rep, 14, 419-433 (2022)

PubMed: 36278554