COX2 Antibody

• Catalog Number: orb411851
• Category: Antibodies
• Description: The COX2 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide of human COX2 .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide of human COX2
• UniProt ID: P00405, P00403, P00406
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: COII; COXII; MTCO2; Cytochrome c oxidase subunit 2
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 17709, 4513

Western blot analysis of COX2 expression in mouse liver (A), mouse heart (B), rat liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 23 kD)

Immunohistochemical analysis of COX2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.