COX2 Antibody

• Catalog Number: orb411834
• Category: Antibodies
• Description: The COX2 Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide of human COX2 .Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide of human COX2
• UniProt ID: P00405, P00403, P00406
• Tested applications: IF, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: COII; COXII; MTCO2; Cytochrome c oxidase subunit 2
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 17709, 4513

Western blot analysis of COX2 expression in mouse heart (A), mouse kidney (B), rat liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)

Immunofluorescent analysis of COX2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).