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Anti-COX2 Antibody

Catalog Number: orb411834

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb411834
CategoryAntibodies
DescriptionRabbit polyclonal antibody to MT-CO2
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic peptide of human COX2
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-2000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetMT-CO2
Entrez4513, 17709
UniProt IDP00405, P00403, P00406
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti-COII antibody, anti-COXII antibody, anti-MTCO
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-COX2 Antibody

Western blot analysis of COX2 expression in mouse heart (A), mouse kidney (B), rat liver (C), rat kidney (D) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)

Anti-COX2 Antibody

Immunofluorescent analysis of COX2 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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