COL4A2 Antibody

SKU: orb865616

Description

Anti-COL4A2 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Human.

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: IHC: 1:50-400; ELISA: 1:100~1000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human COL4A2 recombinant protein (Position: H1666-L1712).
• Target: Collagen alpha-2
• Molecular Weight: 72 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: PBS, 0.02% NaN3, 1 mg/ml rAlbumin and 50% glycerol
• Concentration: 500 ug/ml
• Disclaimer: For research use only

Alternative Names

COL4A2; Collagen alpha 2(IV) chain; Collagen Type IV

IHC analysis of COL4A2 using anti-COL4A2 antibody. COL4A2 was detected in a paraffin-embedded section of human gastric adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-COL4A2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of COL4A2 using anti-COL4A2 antibody. COL4A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-COL4A2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

Collagen alpha-2

UniProt

P08572