Anti-CHRM4 Antibody

• Catalog Number: orb1421737
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CHRM4.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: CHRM4
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: WB: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500), IF: WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
• Conjugation: Unconjugated
• UniProt ID: P08173
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Muscarinic acetylcholine receptor M4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CHRM4 expression in C6 (A), SGC7901 (B), U87MG (C), A375 (D) whole cell lysates.

Immunofluorescent analysis of CHRM4 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.