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Anti-CENPA (Phospho-S7) Antibody

Catalog Number: orb1289873

DispatchUsually dispatched within 5-10 working days
$ 190.00
Catalog Numberorb1289873
CategoryAntibodies
DescriptionRabbit Polyclonal antibody to CENPA
TargetCENPA
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S7 of human CENPA protein. The exact sequence is proprietary.
UniProt IDP49450
Tested applicationsIF, WB
Dilution rangeWB: 1:500-1:1000, IF:IC: 1:50-1:200
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesHistone H3-like centromeric protein A; Centromere
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NoteFor research use only
Entrez1058
Anti-CENPA (Phospho-S7) Antibody

Western blot analysis of CENPA (Phospho-S7) expression in HEK293T (A), Hela (B), DLD (C), rat testis (D), rat spleen (E) whole cell lysates. (Predicted band size: 15 kD; Observed band size: 17 kD)

Anti-CENPA (Phospho-S7) Antibody

Immunofluorescent analysis of CENPA (Phospho-S7) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Anti-CENPA (Phospho-S7) Antibody

Direct ELISA antibody dose-response curve using Anti-CENPA (Phospho-S7) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.