Cdk2 Rabbit Polyclonal Antibody

SKU: orb308829

Description

Anti-Cdk2 Antibody. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human.

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Protein Biochemistry

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution Range: Western blot, 0.1-0.5 μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.
• Target: Cyclin-dependent kinase 2
• Molecular Weight: 33 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cyclin-dependent kinase 2; 2.7.11.22; Cell division protein kinase 2; p33 protein kinase; CDK2; CDKN2

IF analysis of Cdk2 using anti-Cdk2 antibody and anti-Beta Tubulin antibody. Cdk2 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-Cdk2 Antibody and mouse anti-Beta Tubulin antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG and DyLight®594 Conjugated Goat Anti-Mouse IgG were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IHC analysis of Cdk2 using anti-Cdk2 antibody. Cdk2 was detected in a paraffin-embedded section of human invasive urothelial carcinoma of the bladder with squamous differentiation tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cdk2 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Western blot analysis of Cdk2 using anti-Cdk2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurlat whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human U2OS whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human T47D whole cell lysates, Lane 6: human CACO-2 whole cell lysates, Lane 7: human 293T whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdk2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cdk2 at approximately 30 kDa. The expected band size for Cdk2 is at 30, 33 kDa.

Quick Database Links

Gene Symbol

Cyclin-dependent kinase 2

UniProt

P24941