Anti-CDH6 / K-Cadherin Reference Antibody (HKT288)

• Catalog Number: orb1818465
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG1
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 144.24
• UniProt ID: P55285
• Protein Sequence: CDH6 / K-Cadherin
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Cadherin-6, Kidney cadherin, K-cadherin, CDH6
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The purity of Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) is more than 97.93%, determined by SEC-HPLC.

Immobilized Cyno CDH6 His at 2 µg/mL can bind Anti-CDH6 / K-Cadherin Reference Antibody (HKT288), EC50=0.006944 µg/mL.

HKT288 induced OVCAR3 Luciferase activity was evaluated using huCD16a (V158) -NF-AT-Jurkat Reporter Cell. The EC50 was approximately 0.6792 nM.

Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

The endocytosis ratio HKT288 by Hu-CDH6-HEK293 (A6) increased with the increase of antibody concentration, and the Internalization Rate (%). reached 93% at antibody concentration of 2 nM.

Human CDH6 HEK293 (A6) cells were stained with Anti-CDH6 / K-Cadherin Reference Antibody (HKT288) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC71=0.08624 ug/mL