Anti-CD8a Antibody

• Catalog Number: orb1289739
• Category: Antibodies
• Description: Rabbit Polyclonal antibody to CD8a
• Target: CD8A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant protein of human CD8a. The exact sequence is proprietary.
• UniProt ID: P01732, P01731
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1:1000, IH: 1:50-1:200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MAL; T-cell surface glycoprotein CD8 alpha chain;
• Note: For research use only
• Entrez: 925, 12525

Western blot analysis of CD8a expression in mouse pancreas (A) whole cell lysates. (Predicted band size: 21; 25; 30 kD; Observed band size: 30 kD)

Immunohistochemical analysis of CD8a staining in mouse spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.