CD88 Antibody

• Catalog Number: orb393229
• Category: Antibodies
• Description: Rabbit polyclonal antibody to C5AR1.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD88. The exact sequence is proprietary.
• UniProt ID: P21730, P30993, P97520
• Tested applications: IF, IHC, WB
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200, IF/ICC: 1:100:500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: C5AR; C5R1; C5a anaphylatoxin chemotactic receptor
• Research Area: Epigenetics, GPCR, Neuroscience, Signaling Pathway
• Note: For research use only
• Entrez: 728, 113959
• Expiration Date: 12 months from date of receipt.

References

Mustafa Titiz # 1, Lorenzo Landini # 1, Daniel Souza Monteiro de Araujo # 1, Matilde Marini 1, Viola Seravalli 2, Martina Chieca 1, Pasquale Pensieri 1, Marco Montini 3, Gaetano De Siena 1, Benedetta Pasquini 4, Silvia Vannuccini 5, Luigi Francesco Iannone 1, Thiago M Cunha 6, Giulia Brancolini 7, Elisa Bellantoni 1, Irene Scuffi 1, Alessandra Mastricci 1, Martina Tesi 1, Mariarosaria Di Tommaso 2, Felice Petraglia 5, Pierangelo Geppetti 1 8 9, Romina Nassini 10, Francesco De Logu Schwann cell C5aR1 co-opts inflammasome NLRP1 to sustain pain in a mouse model of endometriosis Nat Commun, (2024)

PubMed: 39587068

Applications

IF

Reactivity

Mouse

Western blot analysis of CD88 expression in A375 (A), HEK293T (B) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 42 kD)

Immunohistochemical analysis of CD88 staining in human kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CD88 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).