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Anti-CD79a Antibody

Catalog Number: orb666228

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb666228
CategoryAntibodies
DescriptionRabbit polyclonal antibody to CD79a
TargetCD79A
ClonalityPolyclonal
Species/HostRabbit
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ImmunogenRecombinant fusion protein of human CD79a. The exact sequence is proprietary.
UniProt IDP11911, P11912
Tested applicationsIF, IH, WB
Dilution rangeWB: 1-500-2000, IF: 1-50-200
Antibody TypePrimary Antibody
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesAnti-IGA antibody, anti-MB1 antibody, anti-B-cell
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NoteFor research use only
Entrez973, 12518
Anti-CD79a Antibody

Western blot analysis of CD79a expression in Raji (A) whole cell lysates. (Predicted band size: 20; 25 kD; Observed band size: 40-50 kD)

Anti-CD79a Antibody

Immunohistochemical analysis of CD79a staining in rat spleen formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-CD79a Antibody

Immunofluorescent analysis of CD79a staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

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    FACS,  IF,  IHC-P,  WB

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    Recombinant

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    20 μg, 100 μg