Anti-CD47 Reference Antibody (lemzoparlimab)

• Catalog Number: orb1818232
• Category: Antibodies
• Description: Recombinant Antibody
• Clonality: Monoclonal
• Tested applications: ELISA, FC
• Reactivity: Human, Monkey
• Isotype: IgG4
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Form/Appearance: Purified monoclonal antibody supplied in PBS, pH 6.0, without preservative.This antibody is purified through a protein A column.
• Conjugation: Unconjugated
• MW: 145.58
• UniProt ID: Q08722
• Protein Sequence: CD47
• Expression System: CHO Cell
• Endotoxins: < 0.001EU/μg,determined by LAL method.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Leukocyte surface antigen CD47, Antigenic surface
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

The purity of Anti-CD47 Reference Antibody (lemzoparlimab) is more than 100%, determined by SEC-HPLC.

Immobilized Recomvinant human CD47 (c His) at1.5 µg/mL can bind Anti-CD47 Reference Antibody (lemzoparlimab), EC50=0.0163 µg/mL

Anti-CD47 Reference Antibody (lemzoparlimab) FACS Blocking was evaluated using CCRF-CEM. The IC50 was approximately 0.4557 ug/mL.

The phagocytosis ratio lemzoparlimab by CCRF-CEM increased with the increase of antibody concentration, and the phagocytosis Rate (%) reached 43%.

Anti-CD47 Reference Antibody (lemzoparlimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%

PANC-1 cells were stained with Anti-CD47 Reference Antibody (lemzoparlimab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC317=0.265 ug/mL