CD29 Rabbit Polyclonal Antibody

SKU: orb338971

Description

The CD29 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD29. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Reactivity: Bovine, Gallus, Human, Mouse, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CD29. The exact sequence is proprietary.
• Target: ITGB1
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FNRB; MDF2; MSK12; Integrin beta-1; Fibronectin receptor subunit beta; Glycoprotein IIa; GPIIA; VLA-4 subunit beta; CD29

Western blot analysis of CD29 expression in U87MG (A), A375 (B), rat skin (C) whole cell lysates. (Predicted band size: 88 kD; Observed band size: 115 kD)

Immunohistochemical analysis of CD29 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of CD29 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

ITGB1

UniProt

P49134, P05556, P09055

NCBI Gene ID

16412, 3688, 24511

Ozge Ozgenc Cinar et al. Evaluation of ovarian remodeling-associated markers across prepubertal and adult cyclic stages in the dog Theriogenology, (2026)

PubMed: 41719733

Applications

IF

Reactivity

Canine

Kumsal Orkun et al. Expression Patterns of CXCR4, ITGB1, and FGFR3 in Feline Spermatogonial Stem Cells Theriogenology ., (2026)

PubMed:

Applications

IF

Reactivity

Feline