Cd19 Rabbit Polyclonal Antibody

SKU: orb763106

Description

Anti-Cd19 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Rat.

Research Area

Immunology & Inflammation, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Rat ELISA, 0.1-0.5 μg/ml, Rat
• Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived rat Cd19 recombinant protein (Position: D89-L420).
• Target: CD19 molecule
• Molecular Weight: 85 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
• Concentration: 500 µg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

B lymphocyte antigen CD19; B4; CD19; CD19 molecule; Differentiation antigen CD19; T cell surface antigen Leu 12

IHC analysis of Cd19 using anti-Cd19 antibody. Cd19 was detected in a paraffin-embedded section of rat lymph node tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Cd19 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Quick Database Links

Gene Symbol

CD19 molecule

UniProt

F1LNH2