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Anti-CD16/FCGR3A Antibody

Catalog Number: orb402246

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb402246
CategoryAntibodies
DescriptionAnti-CD16/FCGR3A Antibody. Tested in ELISA, Flow Cytometry, ICC/IF, IHC applications. This antibody reacts with Human.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, ICC, IF, IHC
ReactivityHuman
IsotypeRabbit IgG
ImmunogenE. coli-derived human CD16 recombinant protein (Position: Q101-D166).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW45 kDa
UniProt IDP08637
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesLow affinity immunoglobulin gamma Fc region recept
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NoteFor research use only
Application notesImmunohistochemistry (Paraffin-embedded Section), 2-5μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry (Fixed), 1-3μg/1x106 cells ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-CD16/FCGR3A Antibody

Flow Cytometry analysis of human PBMC cells using anti-CD16 antibody. Overlay histogram showing human PBMC cells (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD16 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Anti-CD16/FCGR3A Antibody

IF analysis of CD16 using anti-CD16 antibody. CD16 was detected in an immunocytochemical section of K562 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL rabbit anti-CD16 Antibody overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Anti-CD16/FCGR3A Antibody

IHC analysis of CD16 using anti-CD16 antibody. CD16 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CD16 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-CD16/FCGR3A Antibody

IHC analysis of CD16 using anti-CD16 antibody. CD16 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CD16 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Anti-CD16/FCGR3A Antibody

IHC analysis of CD16 using anti-CD16 antibody. CD16 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-CD16 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

  • Anti-CD16/FCGR3A Antibody [orb2613026]

    ELISA,  FC,  ICC,  IF,  IHC

    Human

    Rabbit

    Polyclonal

    iFluor647

    100 μg
  • Anti-CD16/FCGR3A Antibody [orb2613027]

    ELISA,  FC,  ICC,  IF,  IHC

    Human

    Rabbit

    Polyclonal

    PE

    100 μg
  • Anti-CD16/FCGR3A Antibody [orb2613028]

    ELISA,  FC,  ICC,  IF,  IHC

    Human

    Rabbit

    Polyclonal

    APC

    100 μg
  • Anti-CD16/FCGR3A Antibody [orb2613029]

    ELISA,  FC,  ICC,  IF,  IHC

    Human

    Rabbit

    Polyclonal

    HRP

    100 μg
  • Anti-CD16/FCGR3A Antibody [orb2613030]

    ELISA,  FC,  ICC,  IF,  IHC

    Human

    Rabbit

    Polyclonal

    FITC

    100 μg