CD163b Antibody

• Catalog Number: orb1880430
• Category: Antibodies
• Description: The CD163b Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CD163b. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CD163b. The exact sequence is proprietary.
• UniProt ID: Q9NR16
• Tested applications: IHC, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD163B; M160; Scavenger receptor cysteine-rich typ
• Note: For research use only
• Entrez: 283316

Western blot analysis of CD163b expression in U2OS (A) whole cell lysates. (Predicted band size: 159 kD; Observed band size: 180 kD)

Immunohistochemical analysis of CD163b staining in human lymph node formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.