You have no items in your shopping cart.
Cart summary
Item 1 of 2
Item 1 of 2
Anti-CD153/TNFSF8 Antibody
Catalog Number: orb334482
| Catalog Number | orb334482 |
|---|---|
| Category | Antibodies |
| Description | Anti-CD153/TNFSF8 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, IHC |
| Reactivity | Human |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153. |
| Antibody Type | Primary Antibody |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 86 kDa |
| UniProt ID | P32971 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Tumor necrosis factor ligand superfamily member 8; Read more... |
| Note | For research use only |
| Application notes | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/mlFlow Cytometry, 1-3μg/1x106cells. Add 0.2ml of distilled water will yield a concentration of 500ug/ml |
| Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of U937 cells using anti-CD153 antibody. Overlay histogram showing U937 cells (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD153 Antibody (1 µg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
CD153 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti-CD153 Antigen Affinity purified polyclonal antibody at 1 µg/mL. The immunohistochemical section was developed using SABC method.
