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Anti-CD134/OX40/Tnfrsf4 Antibody

Catalog Number: orb413088

DispatchUsually dispatched within 3-4 weeks
$ 210.00
Catalog Numberorb413088
CategoryAntibodies
DescriptionAnti-CD134/OX40/Tnfrsf4 Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityMouse, Rat
IsotypeRabbit IgG
ImmunogenE. coli-derived rat TNFRSF4 recombinant protein (Position: V20-L198).
Antibody TypePrimary Antibody
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form/AppearanceLyophilized
ConjugationUnconjugated
MW45 kDa
UniProt IDP15725
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative namesTumor necrosis factor receptor superfamily member
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NoteFor research use only
Application notesWestern blot, 0.1-0.5μg/ml ELISA, 0.1-0.5μg/ml. Add 0.2ml of distilled water will yield a concentration of 500ug/ml
Expiration Date12 months from date of receipt.
Anti-CD134/OX40/Tnfrsf4 Antibody

Western blot analysis of CD134/OX40 using anti-CD134/OX40 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134/OX40 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD134/OX40 at approximately 45KD. The expected band size for CD134/OX40 is at 29KD.

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    FC,  ICC,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
  • Anti-CD134/OX40/TNFRSF4 Antibody [orb107638]

    WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    10 μg, 100 μg
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    WB

    Mouse

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 10 μg
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    WB

    Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μg, 10 μg
  • Anti-CD134/OX40/TNFRSF4 Antibody [orb2575124]

    WB

    Rat

    Rabbit

    Polyclonal

    iFluor647

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