Anti-CD11c [OX-42]

• Catalog Number: orb2645740
• Category: Antibodies
• Description: Anti-CD11c [OX-42]
• Target: CD11c
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: Resident peritoneal cells from F1 hybrids between PVG-RT1c and PVG-RTu and between PVG-RT1c and DA-RT1a rats.
• Tested applications: FC, IHC, IP
• Clone Number: OX-42
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: iC3b receptor; Integrin alpha X; complement compon
• Note: For research use only

Flow-cytometry using anti-CD11c (Integrin alpha) antibody OX42 Staining of rat (Rattus norvegicus) leukocytes with either an isotype control (A) or the rabbit-chimeric version of OX-42 (B, orb2645740) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using an Alexa Fluor ® 488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed rat splenocyte cells with anti-CD11c (Integrin alpha) antibody OX-42. Immunofluorescence analysis of paraformaldehyde fixed rat (Rattus norvegicus) splenocyte cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of OX-42 (orb2645740) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb2645740, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.