CD11b/ITGAM Rabbit Polyclonal Antibody

SKU: orb413002

Description

The CD11b/ITGAM Rabbit Polyclonal Antibody is an unconjugated antibody raised in rabbit. It is immunogen affinity purified and suitable for use in ELISA, FC, IHC, and WB applications. This antibody targets the Integrin alpha-M in Human and Mouse samples.

Research Area

Cell Biology, Disease Biomarkers, Immunology & Inflammation, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human ELISA, 0.1-0.5μg/ml
• Reactivity: Human, Mouse
• Application Notes:
  For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human CD11b recombinant protein (Position: N129-E336).
• Target: Integrin alpha-M
• Molecular Weight: 179 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CD11B; CR 3 alpha chain; CR3A; Integrin alpha M; ITGAM; MAC 1; MAC1A; MO1A; Neutrophil adherence receptor; SLEB6

Quick Database Links

Gene Symbol

Integrin alpha-M

UniProt

P11215