CBL Antibody

SKU: orb196248

Description

CBL Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CBL recombinant protein (Position: A556-T906). Human CBL shares 84% amino acid (aa) sequence identity with mouse CBL.
• Target: E3 ubiquitin-protein ligase CBL
• Molecular Weight: 120 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains 5mg rAlbumin, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

E3 ubiquitin-protein ligase CBL; 6.3.2.-; Casitas B-lineage lymphoma proto-oncogene; Proto-oncogene c-Cbl; RING finger protein 55; Signal transduction protein CBL; CBL; CBL2, RNF55

Flow Cytometry analysis of A549 cells using anti-CBL antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CBL using anti-CBL antibody.Lane 1:human HeLa cell; 2:human MCF-7 cell; 3:human HepG2 cell; 4:human A549 cell; 5:rat RH35 cell; 6:mouse Ana-1 cell; 7:mouse NIH/3T3 cell.

IF analysis of CBL using anti-CBL antibody. CBL was detected in immunocytochemical section of U20S cells.

IHC analysis of CBL using anti-CBL antibody. CBL was detected in paraffin-embedded section of human intestinal cancer tissues.

IHC analysis of CBL using anti-CBL antibody. CBL was detected in paraffin-embedded section of mouse brain tissues.

IHC analysis of CBL using anti-CBL antibody. CBL was detected in paraffin-embedded section of rat brain tissues.

Quick Database Links

Gene Symbol

E3 ubiquitin-protein ligase CBL

UniProt

P22681