Cathepsin L/MEP/CTSL Antibody

• Catalog Number: orb1098011
• Category: Antibodies
• Description: Anti-Cathepsin L/MEP/CTSL Antibody. Tested in WB applications. This antibody reacts with Human, Mouse.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunogen affinity purified.
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cathepsin L/MEP/CTSL, which shares 61.1% amino acid (aa) sequence identity with rat CTSL.
• UniProt ID: P07711
• MW: 37 kDa
• Tested applications: WB
• Application notes: Western blot, 0.25-0.5 μg/ml, Human, Mouse. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Cross Reactivity: No cross-reactivity with other proteins.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Neutrophil cytosol factor 1; NCF-1; 47 kDa autosom
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Cathepsin L/MEP/CTSL using anti-Cathepsin L/MEP/CTSL antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCT tissue lysates, Lane 3: human HCCP tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin L/MEP/CTSL antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cathepsin L/MEP/CTSL at approximately 37 kDa. The expected band size for Cathepsin L/MEP/CTSL is at 37 kDa.